It was confirmed before the Six Nations that centre Tatyana Heard would miss the entire competition with a foot injury.
And on Monday scrum-half Natasha Hunt and lock Morwenna Talling were ruled out of the rest of the tournament after suffering leg injuries against Ireland.
There were already several absentees from the squad that won the World Cup in September.
Zoe Stratford, Abbie Ward, Rosie Galligan and Lark Atkin-Davies are pregnant, while Emily Scarratt and Abby Dow have retired.
