Gloucester have three Wales starters with Anscombe joined by scrum-half Tomos Williams and centre Max Llewellyn.
Wing Josh Hathaway and lock Freddie Thomas are also in the squad, while forwards coach Rhys Thomas has been involved in the backroom staff.
England will arrive at the Principality Stadium as title contenders, although a bonus-point France victory against Scotland later on Saturday night will guarantee Les Bleus top spot.
Anscombe admits a Wales win would give him some bragging rights when the players return to Gloucester.
“It would make going into training a bit more enjoyable,” said Anscombe.
“You don’t need to build this game up and England are coming here with a chance to do something as well.
“England in the stadium is always a bit different. We know there’s going to be an amazing buzz and we would all want to give the fans something to cheer about.”
Anscombe’s future at Kingsholm is uncertain after only one season, with Ireland fly-half Ross Byrne signing for Gloucester.
“The Six Nations is such a unique competition and having come in halfway through, it’s been so full-on for the last three weeks we’re just trying to play the game and what needs to change,” said Anscombe.
“My only focus has been on enjoying pulling on the red jersey because when you get to my age you don’t know how many times you are going to get a chance.
“For me it’s just enjoying the moment, playing for Wales and I will take care of the future in the next few weeks.”
